The following mouse models developed by the Rosen lab are available for purchase from Jackson Laboratory:
010803 – B6;FVB-Tg(Adipoq-cre)1Evdr/J
Mice harboring the Adipoq-Cre BAC transgene express Cre recombinase under control of the mouse adiponectin (Adipoq) promoter/enhancer regions within the BAC transgene. Adipoq-Cre BAC transgenic mice are a Cre-lox tool for deletion of floxed sequences in white adipose tissue and brown adipose tissue, but not in activated or resident macrophages.
A C57BL/6J congenic version of this strain is available as Stock No. 028020.
Citation for this mouse:
Eguchi J; Wang X; Yu S; Kershaw EE; Chiu PC; Dushay J; Estall JL; Klein U; Maratos-Flier E; Rosen ED. 2011. Transcriptional Control of Adipose Lipid Handling by IRF4. Cell Metab 13(3):249-59. doi: 10.1016/j.cmet.2011.02.005.
024670 – B6.FVB-Tg(Ucp1-cre)1Evdr/J
These Ucp1-cre mice express Cre recombinase in interscapular brown fat at room temperature, and in inguinal and epididymal white adipose tissue after cold exposure.
Citation for this mouse:
Kong X, Banks A, Liu T, Kazak L, Rao RR, Cohen P, Wang X, Yu S, Lo JC, Tseng YH, Cypess AM, Xue R, Kleiner S, Kang S, Spiegelman BM, Rosen ED. 2014. IRF4 is a key thermogenic transcriptional partner of PGC-1α. Cell 158(1):69-83. doi: 10.1016/j.cell.2014.04.049.
024671 – B6.129-Tg(Adipoq-cre/Esr1*)1Evdr/J
These transgenic mice express a tamoxifen-inducible cre recombinase from a Adipoq promoter. They may be useful in generating adipocyte-specific targeted mutants in studies related to adipose tissue function and storage, obesity, and other metabolic diseases.
Citation for this mouse:
Jeffery E, Berry R, Church CD, Yu S, Shook BA, Horsley V, Rosen ED, Rodeheffer MS. 2014. Characterization of Cre recombinase models for the study of adipose tissue. Adipocyte 3(3):206-11. doi: 10.4161/adip.29674.
029899 – B6;129S6-Gt(ROSA)26Sortm2(CAG-NuTRAP)Evdr/J
The NuTRAP allele (Nuclear tagging and Translating Ribosome Affinity Purification) has a loxP-flanked STOP preventing transcription of three individual components: BirA, BLRP-tagged mCherry/mRANGAP1 and EGFP/L10a. When expressed, the BLRP-tagged mCherry/mRANGAP1 protein is biotinylated by BirA, allowing nuclear membrane-labeling with mCherry and biotin (which enables nuclear isolation by fluorescence- and affinity-based purification). Furthermore, EGFP/L10a fluorescently tags the translating mRNA polysome complex (which enables isolation of RNAs that are actively engaged by ribosomes). Overall, NuTRAP mice are a Cre-inducible tool strain that allows labeling and simultaneous isolation of cell type-specific nuclei and mRNA, and are well-suited for studying epigenomics and transcriptomics from specific cell types within a heterogeneous tissue. Of note, the donating investigator also has available their similar mouse line H2B-TRAP (Stock No. 029789).
Citation for this mouse:
Roh HC, Tsai LT, Lyubetskaya A, Tenen D, Kumari M, Rosen ED. 2017. Simultaneous Transcriptional and Epigenomic Profiling from Specific Cell Types within Heterogeneous Tissues In Vivo. Cell Rep 18(4):1048-1061. doi: 10.1016/j.celrep.2016.12.087.
029789 – B6.Cg-Gt(ROSA)26Sortm1(CAG-HIST1H2BJ/mCherry,-EGFP/Rpl10a)Evdr/J
The H2B-TRAP allele (Translating Ribosome Affinity Purification) has a loxP-flanked STOP preventing transcription of two individual components: H2B/mCherry and EGFP/L10a. When expressed, the H2B/mCherry protein allows nuclei-labeling with mCherry (which enables nuclear isolation by fluorescence-based purification). Furthermore, EGFP/L10a fluorescently tags the translating mRNA polysome complex (which enables isolation of RNAs that are actively engaged by ribosomes). Overall, H2B-TRAP mice are a Cre-inducible tool strain that allows labeling and simultaneous isolation of cell type-specific nuclei and mRNA, and are well-suited for studying epigenomics and transcriptomics from specific cell types within a heterogeneous tissue. Of note, the donating investigator also has available their similar mouse line NuTRAP (Stock No. 029899).
Citation for this mouse:
Roh HC, Tsai LT, Lyubetskaya A, Tenen D, Kumari M, Rosen ED. 2017. Simultaneous Transcriptional and Epigenomic Profiling from Specific Cell Types within Heterogeneous Tissues In Vivo. Cell Rep 18(4):1048-1061. doi: 10.1016/j.celrep.2016.12.087.